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cell suspension  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cell suspension
    Cell Suspension, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2096 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+suspensions/Neural+Tissue+Dissociation+Kit/pm42844257-299-19-34
    Average 97 stars, based on 2096 article reviews
    cell suspension - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Single Cell:

    Article Title: Endothelial cell, but not neutrophil, programmed cell death receptor-ligand 1 loss has a morbid impact on experimental murine shock/sepsis-induced lung injury
    Article Snippet: .. Briefly, single-cell suspensions of lung tissue were prepared using a mouse lung dissociation kit (Miltenyi Biotec; cat#: 130-095-927) and gentleMACSTM dissociator as per the manufacturer’s protocol (Miltenyi Biotec, Auburn, CA) ( ). ..

    Article Title: Circadian misalignment underlies immune escape in breast cancer
    Article Snippet: Digested tissues were passed through a 70 μm cell strainer, washed, and treated with red blood cell lysis buffer (Thermo Fisher Scientific, #BDB555899). .. After neutralization and additional washes, single-cell suspensions were incubated with CD45 MicroBeads (Miltenyi Biotec, #130-052-301) according to the manufacturer’s protocol. ..

    Article Title: Miniaturized IL-2/anti-IL-2 immunocytokines selectively activate and support the in vivo persistence of regulatory T cells
    Article Snippet: .. Single-cell suspensions were prepared by homogenization (GentleMACS Dissociator, Miltenyi Biotec) and subjected to ACK red blood cell lysis buffer (Gibco). ..

    Article Title: Selective cross-TAD gene regulation by the Tcra enhancer during T cell development
    Article Snippet: Thymocytes and splenocytes were obtained by mechanical dissociation of the thymus and spleen through 70-μm nylon mesh strainers (Pluriselect, 43-50070-51). .. Single-cell suspensions from liver, kidney, and testis extracts were prepared using a gentleMACS Dissociator (Miltenyi Biotech) according to the manufacturer’s instructions. ..

    Article Title: BCG vaccination mitigates tau pathology and restores cognitive function in PS19 mice
    Article Snippet: Briefly, the mice were euthanized via isoflurane overdose, their brains were harvested following complete transcardial perfusion with 15 mL of chilled 1X D-PBS (ThermoFisher scientific, Cat. No.- 14287072), washed in D-PBS, cut into small pieces using scalpel, and collected in 1950 μl Enzyme mix 1 + 30 μl Enzyme Mix 2 containing gentleMACS C-tubes. .. The cut-tissue was dissociated using the gentleMACS Octo Dissociator (program-37C_ABDK_01), and single-cell suspensions were obtained by filtering dissociated tissue through 100 μm MACS Smartstrainers (Miltenyi Biotec, Cat. No.-130-110-916). ..

    Article Title: A Neuroimmune Feedforward Circuit Linking REM Sleep with Stress-Related Behavioral Susceptibility
    Article Snippet: Library preparation, sequencing, and primary bioinformatic processing were performed by OE Biotech Co., Ltd. (Shanghai, China). .. For bulk RNA sequencing of splenic CD11b+ cells from Cx3cr1–Adrb2 conditional knockout mice, single-cell suspensions were prepared from spleens and CD11b+ cells were enriched by positive magnetic selection using the MACS system (Miltenyi Biotec, 130-126-725) prior to RNA extraction and sequencing. .. Total RNA was isolated from brain tissue, peripheral blood mononuclear cells (PBMCs), and spleen using Freezol Reagent (Vazyme) according to the manufacturer’s instructions.

    Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair.
    Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany). .. At least 500,000 live (DAPI-) lineage negative (CD45,CD31-,CD326-) cells from 3 mice per line and time point, except for the 8-week Plin2 group which has 2 mice, were collected per population on the FACSAria Fusion (BD Biosciences) (Supplemental Figure 9).

    Article Title: Metastasis enables immunogenicity through migrasome-mediated antigen release.
    Article Snippet: The tissue was digested for Nature Immunology Article https://doi.org/10.1038/s41590-026-02641-0 30 min at 37 °C in a digestion mixture containing 1 mg ml−1 collagenase I (C8140, Solarbio) and collagenase IV (C8160, Solarbio). .. After digestion, single-cell suspensions were obtained, and tumor-associated cells, including lymphocytes and fibroblasts, were depleted using a Mouse Tumor Cell Isolation Kit (130-110-187, Miltenyi Biotec), to enrich for tumor cells. ..

    Neutralization:

    Article Title: Circadian misalignment underlies immune escape in breast cancer
    Article Snippet: Digested tissues were passed through a 70 μm cell strainer, washed, and treated with red blood cell lysis buffer (Thermo Fisher Scientific, #BDB555899). .. After neutralization and additional washes, single-cell suspensions were incubated with CD45 MicroBeads (Miltenyi Biotec, #130-052-301) according to the manufacturer’s protocol. ..

    Incubation:

    Article Title: Circadian misalignment underlies immune escape in breast cancer
    Article Snippet: Digested tissues were passed through a 70 μm cell strainer, washed, and treated with red blood cell lysis buffer (Thermo Fisher Scientific, #BDB555899). .. After neutralization and additional washes, single-cell suspensions were incubated with CD45 MicroBeads (Miltenyi Biotec, #130-052-301) according to the manufacturer’s protocol. ..

    Homogenization:

    Article Title: Miniaturized IL-2/anti-IL-2 immunocytokines selectively activate and support the in vivo persistence of regulatory T cells
    Article Snippet: .. Single-cell suspensions were prepared by homogenization (GentleMACS Dissociator, Miltenyi Biotec) and subjected to ACK red blood cell lysis buffer (Gibco). ..

    Red Blood Cell Lysis:

    Article Title: Miniaturized IL-2/anti-IL-2 immunocytokines selectively activate and support the in vivo persistence of regulatory T cells
    Article Snippet: .. Single-cell suspensions were prepared by homogenization (GentleMACS Dissociator, Miltenyi Biotec) and subjected to ACK red blood cell lysis buffer (Gibco). ..

    Tissue:

    Article Title: BCG vaccination mitigates tau pathology and restores cognitive function in PS19 mice
    Article Snippet: Briefly, the mice were euthanized via isoflurane overdose, their brains were harvested following complete transcardial perfusion with 15 mL of chilled 1X D-PBS (ThermoFisher scientific, Cat. No.- 14287072), washed in D-PBS, cut into small pieces using scalpel, and collected in 1950 μl Enzyme mix 1 + 30 μl Enzyme Mix 2 containing gentleMACS C-tubes. .. The cut-tissue was dissociated using the gentleMACS Octo Dissociator (program-37C_ABDK_01), and single-cell suspensions were obtained by filtering dissociated tissue through 100 μm MACS Smartstrainers (Miltenyi Biotec, Cat. No.-130-110-916). ..

    Magnetic Cell Separation:

    Article Title: BCG vaccination mitigates tau pathology and restores cognitive function in PS19 mice
    Article Snippet: Briefly, the mice were euthanized via isoflurane overdose, their brains were harvested following complete transcardial perfusion with 15 mL of chilled 1X D-PBS (ThermoFisher scientific, Cat. No.- 14287072), washed in D-PBS, cut into small pieces using scalpel, and collected in 1950 μl Enzyme mix 1 + 30 μl Enzyme Mix 2 containing gentleMACS C-tubes. .. The cut-tissue was dissociated using the gentleMACS Octo Dissociator (program-37C_ABDK_01), and single-cell suspensions were obtained by filtering dissociated tissue through 100 μm MACS Smartstrainers (Miltenyi Biotec, Cat. No.-130-110-916). ..

    Article Title: A Neuroimmune Feedforward Circuit Linking REM Sleep with Stress-Related Behavioral Susceptibility
    Article Snippet: Library preparation, sequencing, and primary bioinformatic processing were performed by OE Biotech Co., Ltd. (Shanghai, China). .. For bulk RNA sequencing of splenic CD11b+ cells from Cx3cr1–Adrb2 conditional knockout mice, single-cell suspensions were prepared from spleens and CD11b+ cells were enriched by positive magnetic selection using the MACS system (Miltenyi Biotec, 130-126-725) prior to RNA extraction and sequencing. .. Total RNA was isolated from brain tissue, peripheral blood mononuclear cells (PBMCs), and spleen using Freezol Reagent (Vazyme) according to the manufacturer’s instructions.

    RNA Sequencing:

    Article Title: A Neuroimmune Feedforward Circuit Linking REM Sleep with Stress-Related Behavioral Susceptibility
    Article Snippet: Library preparation, sequencing, and primary bioinformatic processing were performed by OE Biotech Co., Ltd. (Shanghai, China). .. For bulk RNA sequencing of splenic CD11b+ cells from Cx3cr1–Adrb2 conditional knockout mice, single-cell suspensions were prepared from spleens and CD11b+ cells were enriched by positive magnetic selection using the MACS system (Miltenyi Biotec, 130-126-725) prior to RNA extraction and sequencing. .. Total RNA was isolated from brain tissue, peripheral blood mononuclear cells (PBMCs), and spleen using Freezol Reagent (Vazyme) according to the manufacturer’s instructions.

    Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair.
    Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany). .. At least 500,000 live (DAPI-) lineage negative (CD45,CD31-,CD326-) cells from 3 mice per line and time point, except for the 8-week Plin2 group which has 2 mice, were collected per population on the FACSAria Fusion (BD Biosciences) (Supplemental Figure 9).

    Knock-Out:

    Article Title: A Neuroimmune Feedforward Circuit Linking REM Sleep with Stress-Related Behavioral Susceptibility
    Article Snippet: Library preparation, sequencing, and primary bioinformatic processing were performed by OE Biotech Co., Ltd. (Shanghai, China). .. For bulk RNA sequencing of splenic CD11b+ cells from Cx3cr1–Adrb2 conditional knockout mice, single-cell suspensions were prepared from spleens and CD11b+ cells were enriched by positive magnetic selection using the MACS system (Miltenyi Biotec, 130-126-725) prior to RNA extraction and sequencing. .. Total RNA was isolated from brain tissue, peripheral blood mononuclear cells (PBMCs), and spleen using Freezol Reagent (Vazyme) according to the manufacturer’s instructions.

    Selection:

    Article Title: A Neuroimmune Feedforward Circuit Linking REM Sleep with Stress-Related Behavioral Susceptibility
    Article Snippet: Library preparation, sequencing, and primary bioinformatic processing were performed by OE Biotech Co., Ltd. (Shanghai, China). .. For bulk RNA sequencing of splenic CD11b+ cells from Cx3cr1–Adrb2 conditional knockout mice, single-cell suspensions were prepared from spleens and CD11b+ cells were enriched by positive magnetic selection using the MACS system (Miltenyi Biotec, 130-126-725) prior to RNA extraction and sequencing. .. Total RNA was isolated from brain tissue, peripheral blood mononuclear cells (PBMCs), and spleen using Freezol Reagent (Vazyme) according to the manufacturer’s instructions.

    RNA Extraction:

    Article Title: A Neuroimmune Feedforward Circuit Linking REM Sleep with Stress-Related Behavioral Susceptibility
    Article Snippet: Library preparation, sequencing, and primary bioinformatic processing were performed by OE Biotech Co., Ltd. (Shanghai, China). .. For bulk RNA sequencing of splenic CD11b+ cells from Cx3cr1–Adrb2 conditional knockout mice, single-cell suspensions were prepared from spleens and CD11b+ cells were enriched by positive magnetic selection using the MACS system (Miltenyi Biotec, 130-126-725) prior to RNA extraction and sequencing. .. Total RNA was isolated from brain tissue, peripheral blood mononuclear cells (PBMCs), and spleen using Freezol Reagent (Vazyme) according to the manufacturer’s instructions.

    Sequencing:

    Article Title: A Neuroimmune Feedforward Circuit Linking REM Sleep with Stress-Related Behavioral Susceptibility
    Article Snippet: Library preparation, sequencing, and primary bioinformatic processing were performed by OE Biotech Co., Ltd. (Shanghai, China). .. For bulk RNA sequencing of splenic CD11b+ cells from Cx3cr1–Adrb2 conditional knockout mice, single-cell suspensions were prepared from spleens and CD11b+ cells were enriched by positive magnetic selection using the MACS system (Miltenyi Biotec, 130-126-725) prior to RNA extraction and sequencing. .. Total RNA was isolated from brain tissue, peripheral blood mononuclear cells (PBMCs), and spleen using Freezol Reagent (Vazyme) according to the manufacturer’s instructions.

    FACS:

    Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair.
    Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany). .. At least 500,000 live (DAPI-) lineage negative (CD45,CD31-,CD326-) cells from 3 mice per line and time point, except for the 8-week Plin2 group which has 2 mice, were collected per population on the FACSAria Fusion (BD Biosciences) (Supplemental Figure 9).

    Purification:

    Article Title: Pulmonary fibroblast subsets demonstrate differentially enriched signaling pathways during fibrosis resolution and repair.
    Article Snippet: Cytometry data was acquired with the LSRFortessa (BD Biosciences, San Jose, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). .. Cell sorting, and bulk RNA sequencing and analysis Prior to cell sorting, single cell suspensions were enriched for lineage negative cells by incubating with CD45 (130-052-301), CD31 (130-097-418) and CD326 (130-105-958) MicroBeads and purified off LS columns per manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany). .. At least 500,000 live (DAPI-) lineage negative (CD45,CD31-,CD326-) cells from 3 mice per line and time point, except for the 8-week Plin2 group which has 2 mice, were collected per population on the FACSAria Fusion (BD Biosciences) (Supplemental Figure 9).

    Cell Isolation:

    Article Title: Metastasis enables immunogenicity through migrasome-mediated antigen release.
    Article Snippet: The tissue was digested for Nature Immunology Article https://doi.org/10.1038/s41590-026-02641-0 30 min at 37 °C in a digestion mixture containing 1 mg ml−1 collagenase I (C8140, Solarbio) and collagenase IV (C8160, Solarbio). .. After digestion, single-cell suspensions were obtained, and tumor-associated cells, including lymphocytes and fibroblasts, were depleted using a Mouse Tumor Cell Isolation Kit (130-110-187, Miltenyi Biotec), to enrich for tumor cells. ..



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